Anti-ERK1 antibody (ab227179)
Key features and details
- Rabbit polyclonal to ERK1
- Suitable for: IHC-P, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ERK1 antibody
See all ERK1 primary antibodies -
Description
Rabbit polyclonal to ERK1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ERK1 aa 120-155 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P27361 -
Positive control
- IHC-P: Human placenta tissue; Rat colon tissue. ICC/IF: A431 and SMMC7721 cells. Flow Cyt: Mouse splenocytes.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine)
Aqueous buffered solution -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 antibody (ab227179)
Formalin-fixed, paraffin-embedded human placenta tissue stained for ERK1 using ab227179 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-ERK1 antibody (ab227179)A431 (human epidermoid carcinoma cell line) cells stained for ERK1 (green) using ab227179 at 1/50 dilution in ICC/IF, followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor® 488 conjugated secondary antibody.
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Flow Cytometry - Anti-ERK1 antibody (ab227179)Flow cytometric analysis of mouse splenocytes labeling ERK1 with ab227179 at 1/50 dilution folllowed by a FITC conjugated secondary antibody (green) compared with an isotype control (orange), a secondary only control (light blue) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 antibody (ab227179)Formalin-fixed, paraffin-embedded rat colon tissue stained for ERK1 using ab227179 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-ERK1 antibody (ab227179)SMMC-7721 (human hepatocarcinoma cell line) cells stained for ERK1 (green) using ab227179 at 1/50 dilution in ICC/IF, followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor® 488 conjugated secondary antibody.
