Anti-ERGIC1 antibody (ab121582)
Key features and details
- Rabbit polyclonal to ERGIC1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ERGIC1 antibody
See all ERGIC1 primary antibodies -
Description
Rabbit polyclonal to ERGIC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: PGNFHVSTHS ATAQPQNPDM THVIHKLSFG DTLQVQNIHG AFNALGGADR LTSNPLASHD YIL, corresponding to internal sequence amino acids 127-189 of Human ERGIC1
-
Positive control
- WB: Transfected HEK293T cells overexpressing ERGIC1 with a C-terminal myc-DDK tag. ICC/IF: Human cell line U-2 OS IHC: Human colon and human duodenum tissue
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-ERGIC1 antibody (ab121582)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121582 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121582)ab121582 at a 1/50 dilution, staining ERGIC1 in glandular cells of paraffin-embedded Human colon tissue by immunohistochemistry. -
Western blot - Anti-ERGIC1 antibody (ab121582)All lanes : Anti-ERGIC1 antibody (ab121582) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T cell lysate
Lane 2 : Lysate from transfected HEK293T cells overexpressing ERGIC1 with a C-terminal myc-DDK tag (~3.1 kDa)
Developed using the ECL technique.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERGIC1 antibody (ab121582)Immunohistochemical staining of Human duodenum shows strong cytoplasmic positivity using ab121582
