Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (ab189333)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E186] to ErbB3 / HER3 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free
    See all ErbB3 / HER3 primary antibodies

  • Description

    Rabbit monoclonal [E186] to ErbB3 / HER3 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises ErbB3/HER3. WB samples: should avoid boiling.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF-7 cell lysate.

  • General notes

    Ab189333 is the carrier-free version of ab32121. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab189333 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (ab189333)
    Western blot - Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (ab189333)

    This data was developed using ab32121, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: ERBB3 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: MCF7 whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab32121 observed at 185 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab32121 was shown to specifically recognize ERBB3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when knock out samples were examined. Wild-type and ERBB3 knockout samples were subjected to SDS-PAGE. ab32121 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (ab189333)
    Western blot - Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (ab189333)
    Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 1/1000 dilution + MDA-MB-435S (human ductal carcinoma) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 149 kDa



    This data was developed using ab32121, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (ab189333)
    Western blot - Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (ab189333)
    All lanes : Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 2.685 µg/ml (purified)

    Lane 1 : MDA-MB-435S (human mammary gland ductal carcinoma melanocyte) whole cell lysate
    Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 149 kDa


    Exposure time: 37 seconds


    This data was developed using ab32121, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (ab189333)
    Western blot - Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (ab189333)
    Anti-ErbB3 / HER3 antibody [E186] (ab32121) at 1/500 dilution (unpurified) + MCF-7 cell lysate

    Predicted band size: 149 kDa
    Observed band size: 185 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab32121, the same antibody clone in a different buffer formulation.

     

  • Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (ab189333)
    Anti-ErbB3 / HER3 antibody [E186] - BSA and Azide free (ab189333)

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