Anti-EPX antibody (ab65319)
Key features and details
- Rabbit polyclonal to EPX
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-EPX antibody
See all EPX primary antibodies -
Description
Rabbit polyclonal to EPX -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide within Human EPX aa 400-500 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
(Peptide available asab104963) -
Positive control
- This antibody gave a positive signal in the following human tissue lysates: Bone Marrow; Thymus; Spleen.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 81 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 81 kDa).Target
-
Function
Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. -
Involvement in disease
Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD) [MIM:261500]. EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Cytoplasmic granule. Cytoplasmic granules of eosinophils. - Information by UniProt
-
Database links
- Entrez Gene: 8288 Human
- Entrez Gene: 13861 Mouse
- Entrez Gene: 303414 Rat
- Omim: 131399 Human
- SwissProt: P11678 Human
- SwissProt: P49290 Mouse
- Unigene: 279259 Human
- Unigene: 1315 Mouse
-
Alternative names
- Eosinophil peroxidase [Precursor] antibody
- Eosinophil peroxidase antibody
- Eosinophil peroxidase heavy chain antibody
see all
Images
-
Western blot - Anti-EPX antibody (ab65319)All lanes : Anti-EPX antibody (ab65319) at 1 µg/ml
Lane 1 : Human Bone Marrow lysate
Lane 2 : Human Thymus lysate
Lane 3 : Human Spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab65319 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Datasheets and documents
References (0)
ab65319 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-EPX antibody (ab65319)All lanes : Anti-EPX antibody (ab65319) at 1 µg/ml
Lane 1 : Human Bone Marrow lysate
Lane 2 : Human Thymus lysate
Lane 3 : Human Spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab65319 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
