Anti-Epsin 2 antibody (ab242044)
Key features and details
- Rabbit polyclonal to Epsin 2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Epsin 2 antibody
See all Epsin 2 primary antibodies -
Description
Rabbit polyclonal to Epsin 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human Epsin 2 aa 150-200. The exact sequence is proprietary.
Database link: O95208 -
Positive control
- IP: Epsin 2 IP in HeLa whole cell lysate.
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General notes
ab242044 has not performed satisfactorily when used for WB of Epsin 2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Epsin 2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Epsin 2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Epsin 2 antibody (ab242044)
Epsin 2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242044 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab242044 at 0.4 µg/ml.
Lane 1: ab242044 IP in HeLa whole cell lysate.
lane 2: Control IgG in HeLa whole cell lysate.Detection: Chemiluminescence with an exposure time of 30 seconds.
Prepared using NETN lysis buffer.
