Lanes 1-3 : Anti-ENT1 antibody [EPR20556] (ab223851) at 1/5000 dilution
Lanes 4-5 : Anti-ENT1 antibody [EPR20556] (ab223851) at 1/1000 dilution

Lane 1 : MDA-MB-435S (human ductal carcinoma cell line) whole cell lysate at 10 µg
Lane 2 : U-937 (human histiocytic lymphoma cell line) whole cell lysate at 10 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 4 : Human fetal liver tissue lysate at 20 µg
Lane 5 : Human fetal spleen tissue lysate at 20 µg

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 4-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Developed using the ECL technique.

Predicted band size: 50 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

This data was developed using ab223851, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time : Lanes 1 and 4: 3 minutes; Lane 2: 1 minute; Lanes 3 and 5: 15 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID: 12611914; 10085223).

This data was developed using ab223851, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human gallbladder adenocarcinoma tissue labeling ENT1 with ab223851 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in human gallbladder adenocarcinoma (PMID:26266900) is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using ab223851, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue labeling ENT1 with ab223851 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in pancreatic cancer (PMID:21913012) is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using ab223851, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ENT1 with ab223851 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in mouse liver (PMID: 17761511) is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

This data was developed using ab223851, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling ENT1 with ab223851 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining in rat colon epithelium and endothelium (PMID:17761511) is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.