Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19680] to ENO1 + ENO2 + ENO3
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680]
See all ENO1 + ENO2 + ENO3 primary antibodies
Description

Rabbit monoclonal [EPR19680] to ENO1 + ENO2 + ENO3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged human ENO1 (aa2-434) recombinant protein, His-tagged human ENO2 (aa1-434) recombinant protein and His-tagged human ENO3 (aa1-434) recombinant protein. HeLa, Jurkat, NIH/3T3, RAW264.7 and MCF7 whole cell lysate. Human skeletal muscle lysate. Human fetal heart, kidney, brain and spleen lysate. Mouse brain, spleen and kidney lysate. IHC-P: Human pancreas tissue. Human clear cell kidney carcinoma tissue. Mouse pancreas tissue. ICC/IF: HeLa and MCF7 cells. FACS: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19680
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- Other

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514)

All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/1000 dilution

Lane 1 : His-tagged human ENO1 (aa2-434) recombinant protein, 10 ng
Lane 2 : His-tagged human ENO2 (aa1-434) recombinant protein, 10 ng
Lane 3 : His-tagged human ENO3 (aa1-434) recombinant protein, 10 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 47 kDa
why is the actual band size different from the predicted?

Blocking: 5% NFDM/TBST.

Exposure times:

Lanes 1 & 2: 3 seconds

Lane 3: 1 seconds
Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514)

Lanes 1-3 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/2000 dilution
Lane 4 : Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/10000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : Human skeletal muscle
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 47 kDa why is the actual band size different from the predicted?

Blocking: 5% NFDM/TBST.

Exposure times:

Lane 1/2/4: 1 seconds

Lane 3: 3 seconds
Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514)

All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/2000 dilution

Lane 1 : Human fetal heart
Lane 2 : Human fetal kidney
Lane 3 : Human fetal spleen
Lane 4 : Human fetal brain

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 47 kDa why is the actual band size different from the predicted?

Blocking: 5% NFDM/TBST.

Exposure times:

Lane 1-3: 3 seconds

Lane 4: 1 seconds
Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514)

All lanes : Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514) at 1/2000 dilution

Lane 1 : Mouse brain
Lane 2 : Mouse spleen
Lane 3 : Mouse kidney
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 47 kDa why is the actual band size different from the predicted?

Blocking : 5% NFDM/TBST.

Exposure times:

Lane 1, 2 and 4: 3 minutes

Lane 3: 1 minute

Lane 5: 3 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514)

Immunohistochemical analysis of paraffin embedded human pancreas tissue labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on medium-small ducts of human pancreas (PMID: 19425054). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514)

Immunohistochemical analysis of paraffin embedded human clear cell carcinoma tissue labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and nuclear staining on human clear cell kidney carcinoma is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514)

Immunohistochemical analysis of paraffin embedded mouse pancreas tissue labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining on the medium-small ducts of normal mouse pancreas is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514)

Immunofluorescent analysis of 100% methanol fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/500 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line.

Counterstains: ab195889 Anti-alpha Tubulin antibody [DM1A] (red) - Microtubule Marker (Alexa Fluor^® 594) (1/200). DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514)

Immunofluorescent analysis of 100% methanol fixed MCF7 (human breast adenocarcinoma epithelial cell) cells labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/500 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line.

Counterstains: ab195889 Anti-alpha Tubulin antibody [DM1A] (red) - Microtubule Marker (Alexa Fluor^® 594) (1/200). DAPI (blue).
Flow Cytometry - Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514)

Flow cytometric analysis of 4% paraformaldehyde fixed HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling ENO1 + ENO2 + ENO3 with ab222514 at 1/800 (red) compared with an isotype control Rabbit monoclonal IgG (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Secondary antibody Goat anti rabbit IgG Alexa Fluor^® 488 (ab150077), at 1/2000 dilution was used as the secondary antibody.
Anti-ENO1 + ENO2 + ENO3 antibody [EPR19680] (ab222514)

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