Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 09, 2021

Anti-EIF5 antibody [EPR12140(B)] (ab170915)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12140(B)] to EIF5
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-EIF5 antibody [EPR12140(B)]
See all EIF5 primary antibodies
Description

Rabbit monoclonal [EPR12140(B)] to EIF5
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human EIF5 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P55010
Positive control
- K562, HeLa and HepG2 whole cell lysate (ab7900); Human colonic carcinoma and pancreas tissues; K562 and HepG2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12140(B)
Isotype

IgG
Research areas

Western blot - Anti-EIF5 antibody [EPR12140(B)] (ab170915)

All lanes : Anti-EIF5 antibody [EPR12140(B)] (ab170915) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

Predicted band size: 49 kDa
Flow Cytometry - Anti-EIF5 antibody [EPR12140(B)] (ab170915)

Flow cytometric analysis of permeabilized K562 cells labeling EIF5 with ab170915 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Immunocytochemistry/ Immunofluorescence - Anti-EIF5 antibody [EPR12140(B)] (ab170915)

Immunofluorescence analysis of HepG2 cells labeling EIF5 with ab170915 at 1/100 dilution (green). DAPI nuclear staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF5 antibody [EPR12140(B)] (ab170915)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling EIF5 with ab170915 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF5 antibody [EPR12140(B)] (ab170915)

Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling EIF5 with ab170915 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-EIF5 antibody [EPR12140(B)] (ab170915)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com