Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)
![Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)](https://www.abcam.com/ps/products/68/ab68187/Images/ab68187-82384-anti-eif4ebp1-eif4ebp2-eif4ebp3-phospho-t45-antibody-epr2169y-western-blot.jpg "Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2169Y] to eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y]
See all eIF4EBP1 + eIF4EBP2 + eIF4EBP3 primary antibodies -
Description
Rabbit monoclonal [EPR2169Y] to eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
A phospho specific peptide corresponding to residues surrounding threonine 45
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Positive control
- WB: 293T cell lysate IHC-P: Human breast carcinoma tissue
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2169Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)All lanes : Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)
Lane 1 : 293T cell lysate, untreated.
Lane 2 : 293T cell lysate, treated with FBS.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-rabbit at 1/2000 dilution
Predicted band size: 13 kDa
Observed band size: 14-19 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)](https://www.abcam.com/ps/products/68/ab68187/Images/ab68187-82383-anti-eif4ebp1-eif4ebp2-eif4ebp3-phospho-t45-antibody-epr2169y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)Human breast carcinoma tissue stained with ab68187 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)](https://www.abcam.com/ps/products/68/ab68187/Images/ab68187-3-benefits-of-recombinant-antibodies.png)
Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)
