Anti-eIF4E antibody (ab1126)
Key features and details
- Rabbit polyclonal to eIF4E
- Suitable for: WB, IHC-P
- Reacts with: Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-eIF4E antibody
See all eIF4E primary antibodies -
Description
Rabbit polyclonal to eIF4E -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Cow
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Immunogen
Recombinant full length protein (Human).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-eIF4E antibody (ab1126)Anti-eIF4E antibody (ab1126) + 30ug bovine kidney extract
Predicted band size: 25 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Western blot of 30µ g bovine kidney extract with anti-eIF-4E (GLO153). The arrow denotes the position corresponding to eIF-4E (~ 28 kDa). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4E antibody (ab1126)IHC image of ab1126 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1126, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
