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Epigenetics and Nuclear Signaling DNA / RNA Translation tRNAs

Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)

Price and availability

606 422 ₸

Availability

Order now and get it on Thursday June 01, 2023

Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16147] to eIF3g - BSA and Azide free
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-eIF3g antibody [EPR16147] - BSA and Azide free
    See all eIF3g primary antibodies
  • Description

    Rabbit monoclonal [EPR16147] to eIF3g - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251137 is the carrier-free version of ab192601.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR16147
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNAs
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

Images

  • Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)
    Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)
    All lanes : Anti-eIF3g antibody [EPR16147] (ab192601) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : MOLT-4 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab192601, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)
    Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)
    All lanes : Anti-eIF3g antibody [EPR16147] (ab192601) at 1/1000 dilution

    Lane 1 : RAW 264.7 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    This data was developed using ab192601, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)
    Immunocytochemistry - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)
    This data was developed using ab192601, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling eIF3g with ab192601 at a 1/100 dilution ( 7.0 μg/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.
  • Immunoprecipitation - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)
    Immunoprecipitation - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)

    This data was developed using ab192601, the same antibody clone in a different buffer formulation.

    Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling eIF3g using ab192601 at 1/40 dilution and HRP-conjugated anti-rabbit IgG specific to the non-reduced form of rabbit IgG at a 1/1500 dilution.

  • Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)
    Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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