Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23277-60] to Eg5 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, IP, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free
    See all Eg5 primary antibodies

  • Description

    Rabbit monoclonal [EPR23277-60] to Eg5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MCF7 and Jurkat whole cell lysates; Human testis and tonsil tissue lysates. IHC-P: Human tonsil, testis and breast tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate.

  • General notes

    ab270409 is the carrier-free version of ab254299.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Eg5 with ab254299 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in human tonsil (PMID:25277178) is observed.

    The section was incubated with ab254299 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254299).

  • Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)
    Immunocytochemistry/ Immunofluorescence - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell line) cells labelling Eg5 with ab254299 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cells. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254299).

  • Flow Cytometry - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)
    Flow Cytometry - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Eg5 with ab254299 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254299).

  • Immunoprecipitation - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)
    Immunoprecipitation - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)

    Eg5 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab254299 at 1/30 dilution. Western blot was performed on the immunoprecipitate using 254299 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg

    Lane 2: ab254299 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254299 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds

    The minor bands beneath the target band are probably caused by degradation.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254299).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Eg5 with ab254299 at 1/500 dilution (1.18 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in human tonsil (PMID:25277178) is observed.

    The section was incubated with ab254299 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254299).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Eg5 with ab254299 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Cytoplasmic staining in human tonsil (PMID:25277178) is observed. The section was incubated with ab254299 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254299).

  • Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)
    Anti-Eg5 antibody [EPR23277-60] - BSA and Azide free (ab270409)

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