Anti-E2F6 antibody (ab53061)
Key features and details
- Rabbit polyclonal to E2F6
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-E2F6 antibody
See all E2F6 primary antibodies -
Description
Rabbit polyclonal to E2F6 -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total E2F6 protein.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human E2F6 aa 141-190.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-E2F6 antibody (ab53061)All lanes : Anti-E2F6 antibody (ab53061) at 1/500 dilution
Lane 1 : Extracts of HepG2 cells
Lane 2 : Extracts of HepG2 cells treated with peptide
Predicted band size: 31 kDa
Observed band size: 31 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F6 antibody (ab53061)Immunohistochemistry analysis of paraffin-embedded human brain tissue labeling E2F6 with ab53061. The picture on the right is blocked with the synthesized peptide. -
Western blot - Anti-E2F6 antibody (ab53061)Anti-E2F6 antibody (ab53061) at 1/500 dilution + NIH/3T3 cell lysate
Predicted band size: 31 kDa
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Western blot - Anti-E2F6 antibody (ab53061)Anti-E2F6 antibody (ab53061) at 1/500 dilution + HepG2 cell lysate
Predicted band size: 31 kDa
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Western blot - Anti-E2F6 antibody (ab53061) This image is courtesy of an Abreview submitted by Dr. Ligia TavaresAll lanes : Anti-E2F6 antibody (ab53061) at 1/500 dilution (in TBS fro 16 hours at 4°C )
Lane 1 : HeLa cells
Lane 2 : Cell lysate (nuclear) of mouse embryonic stem cells at 100000 cells
Secondary
All lanes : HRP-conjugated Donkey anti rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 31 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Blocking Step: 4% Milk for 1 hour at 22°C
