Anti-E2 tag antibody [5E11] (ab977)
![Anti-E2 tag antibody [5E11] (ab977)](https://www.abcam.com/ps/products/0/ab977/Images/ab977_1.gif "Anti-E2 tag antibody [5E11] (ab977)")
Key features and details
- Mouse monoclonal [5E11] to E2 tag
- Suitable for: IP, WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgG1
Overview
-
Product name
Anti-E2 tag antibody [5E11]
See all E2 tag primary antibodies -
Description
Mouse monoclonal [5E11] to E2 tag -
Host species
Mouse -
Tested applications
Suitable for: IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Epitope
SSTSSDFRDR (linear) -
General notes
E2TAG having sequence SSTSSDFRDR or GVSSTSSDFRDR and antibodies specific for the peptide tag are covered by PCT Patent No PCT/EE01/00001 held by Quattromed Ltd. End-user is granted to use the licenced technology solely for internal research purpose only. This antibody should be used in conjunction with the E2 tagging vectors (links to the appropriate datasheet can be found in the Related Products section at the bottom of the datasheet).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5E11 -
Isotype
IgG1 -
Research areas
Images
-
Western blot - Anti-E2 tag antibody [5E11] (ab977)Cos-7 cell lysates (1
µ g of total protein per lane) were transfected with pQM vectors encoding two different E2-tagged proteins. Detection is performed with anti-E2Tag antibody (5E11) (1:10 000 in TBST) with and without high salt followed by incubation with goat anti-mouse HRP conjugate (1:10,000). Cos-7 cell lysates (1µg of total protein per lane) were transfected with pQM vectors encoding two different E2-tagged proteins. Detection is performed with anti-E2Tag antibody (5E11) (1:10 000 in TBST) with and without high salt followed by incubation with goat anti-mouse HRP conjugate (1:10,000). -
![Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [5E11] (ab977)](https://www.abcam.com/ps/products/0/ab977/Images/ab977-3843-anti-e2-tag-antibody-5e11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [5E11] (ab977) This image is courtesy of an Abreview submitted by Dr Ashraf Mannanab977 at 1/200 dilution staining human Hela cells by ICC/IF. The HeLa cells were transfected with the fusion DNA construct pQM-NTag/A consisting of E2 tag fused in frame with gene specific cDNA. The cells were fixed with paraformaldehyde and blocked with serum prior to incubation with the antibody for 2 hours. A Cy3 conjugated sheep anti-mouse IgG antibody was used as the secondary.
