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Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11244-61] to Dynein intermediate chain 1/DNAI1
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61]
    See all Dynein intermediate chain 1/DNAI1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11244-61] to Dynein intermediate chain 1/DNAI1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Dynein intermediate chain 1/DNAI1 aa 1-200. The exact sequence is proprietary.
    Database link: Q9UI46

  • Positive control

    • Human, rat and mouse testis lysates; Human testis and trachea tissues; Jurkat cells.

  • General notes

     This product was previously labelled as Dynein intermediate chain 1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)
    Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)
    Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Human testis tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 79 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)
    Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)
    Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (purified) + Rat testis lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 79 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)
    Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)
    Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Mouse testis tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 79 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)
    Immunocytochemistry/ Immunofluorescence - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)

    Immunofluorescence staining of F9 cells with purified ab171964 at a working dilution of 1 in 250, counter-stained with DAPI and mouse monoclonal anti-tubulin (ab7291, 1/500). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom right hand and middle panels - for the negative control, purified ab171964 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500.

  • Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)
    Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)
    All lanes : Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (unpurified)

    Lane 1 : Human testis lysate
    Lane 2 : rat testis lysate
    Lane 3 : mouse testis lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 79 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)
    Immunocytochemistry/ Immunofluorescence - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)

    Immunofluorescence analysis of Jurkat cells, labeling Dynein intermediate chain 1/DNAI1 using unpurified ab171964 at a 1/50 dilution (green) and DAPI nuclear staining (blue).

  • Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)
    Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)

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