Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 123 964 ₸

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Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11244-61] to Dynein intermediate chain 1/DNAI1
Suitable for: WB, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61]
See all Dynein intermediate chain 1/DNAI1 primary antibodies
Description

Rabbit monoclonal [EPR11244-61] to Dynein intermediate chain 1/DNAI1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Dynein intermediate chain 1/DNAI1 aa 1-200. The exact sequence is proprietary.
Database link: Q9UI46
Positive control
- Human, rat and mouse testis lysates; Human testis and trachea tissues; Jurkat cells.
General notes
This product was previously labelled as Dynein intermediate chain 1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR11244-61
Isotype

IgG
Research areas

Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)

Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Human testis tissue lysate at 20 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 79 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)

Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (purified) + Rat testis lysate at 20 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 79 kDa
Observed band size: 79 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)

Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Mouse testis tissue lysate at 20 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 79 kDa
Observed band size: 79 kDa

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)

Immunofluorescence staining of F9 cells with purified ab171964 at a working dilution of 1 in 250, counter-stained with DAPI and mouse monoclonal anti-tubulin (ab7291, 1/500). The secondary antibody was Alexa Fluor^® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom right hand and middle panels - for the negative control, purified ab171964 was used at a dilution of 1/200 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500.
Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)

All lanes : Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (unpurified)

Lane 1 : Human testis lysate
Lane 2 : rat testis lysate
Lane 3 : mouse testis lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 79 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)

Immunofluorescence analysis of Jurkat cells, labeling Dynein intermediate chain 1/DNAI1 using unpurified ab171964 at a 1/50 dilution (green) and DAPI nuclear staining (blue).
Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)

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