Anti-DUOX2 antibody (ab97266)
Key features and details
- Rabbit polyclonal to DUOX2
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-DUOX2 antibody -
Description
Rabbit polyclonal to DUOX2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Human, Chimpanzee, Macaque monkey, Orangutan
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Immunogen
Synthetic peptide corresponding to Rat DUOX2 aa 1-100 conjugated to keyhole limpet haemocyanin.
Database link: Q9ES45 -
Positive control
- This antibody gave a positive signal in Rat Colon tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Rat Thyroid.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab97266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Predicted molecular weight: 175 kDa. IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Target
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Function
Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain. -
Tissue specificity
Expressed in colon, small intestine, duodenum and tracheal surface epithelial cells (at protein level). Expressed in thyrocytes. Also detected in kidney, liver, lung, pancreas, prostate, salivary glands, rectum and testis. -
Pathway
Hormone biosynthesis; thyroid hormone biosynthesis. -
Involvement in disease
Defects in DUOX2 are a cause of thyroid dyshormonogenesis 6 (TDH6) [MIM:607200]. A disorder due to a defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. -
Sequence similarities
In the N-terminal section; belongs to the peroxidase family.
Contains 3 EF-hand domains.
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
Developmental stage
Widely expressed in fetal tissues. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Apical cell membrane. Localizes to the apical membrane of epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 50506 Human
- Entrez Gene: 79107 Rat
- Omim: 606759 Human
- SwissProt: Q9NRD8 Human
- SwissProt: Q9ES45 Rat
- Unigene: 71377 Human
- Unigene: 55542 Rat
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Alternative names
- Dual oxidase 2 antibody
- Dual oxidase like domains 2 antibody
- Dual oxidase2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUOX2 antibody (ab97266)
IHC image of DUOX2 staining in Rat thyroid formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97266, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-DUOX2 antibody (ab97266)Anti-DUOX2 antibody (ab97266) at 1 µg/ml (Milk blocking (3%)) + Colon (Rat) Tissue Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 175 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Protocols
Datasheets and documents
References (1)
ab97266 has been referenced in 1 publication.
- Dho SH et al. NOX5-L can stimulate proliferation and apoptosis depending on its levels and cellular context, determining cancer cell susceptibility to cisplatin. Oncotarget 6:39235-46 (2015). PubMed: 26513170
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUOX2 antibody (ab97266)
IHC image of DUOX2 staining in Rat thyroid formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97266, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-DUOX2 antibody (ab97266)Anti-DUOX2 antibody (ab97266) at 1 µg/ml (Milk blocking (3%)) + Colon (Rat) Tissue Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 175 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes