Anti-DUBA2 antibody (ab185352)
Key features and details
- Rabbit polyclonal to DUBA2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-DUBA2 antibody -
Description
Rabbit polyclonal to DUBA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
Immunogen
Recombinant fragment corresponding to Human DUBA2 aa 50-150.
Database link: Q7L8S5 -
Positive control
- IHC-P: Human testis tissue; WB: Transfected HEK-293T whole cell lysates.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUBA2 antibody (ab185352)
Immunohistochemistry analysis of human testis tissue labeling DUBA2 (high expression) with ab185352 at a 1/2500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Western blot - Anti-DUBA2 antibody (ab185352)All lanes : Anti-DUBA2 antibody (ab185352) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : DUBA2 over-expression - HEK-293T whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Predicted band size: 33 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DUBA2 antibody (ab185352)Immunohistochemistry analysis of human endometrium tissue labeling DUBA2 (low expression) with ab185352 at a 1/2500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.