Anti-DOCK9/Trg antibody (ab70272)
Key features and details
- Rabbit polyclonal to DOCK9/Trg
- Suitable for: ICC/IF, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DOCK9/Trg antibody
See all DOCK9/Trg primary antibodies -
Description
Rabbit polyclonal to DOCK9/Trg -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Opossum, Orangutan
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Immunogen
Synthetic peptide, corresponding to amino acids 1-50 of Human DOCK9 (NP_056111.1)
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Positive control
- HeLa whole cell lysate
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DOCK9/Trg antibody (ab70272)All lanes : Anti-DOCK9/Trg antibody (ab70272) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 236 kDa
Observed band size: 236 kDa
Additional bands at: 117 kDa, 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Immunoprecipitation - Anti-DOCK9/Trg antibody (ab70272)HeLa whole cell lysate (1 mg) was immunoprecipitated with ab70272 at 3 µg/mg of lysate (lane 1) or with IgG control (lane 2). Samples were analysed by Western blotting using ab70272 at 0.04 µg/ml and detected by chemiluminescence with a 3 minute exposure time. -
Immunocytochemistry/ Immunofluorescence - Anti-DOCK9/Trg antibody (ab70272)ICC/IF image of ab70272 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70272, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
