Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)
![Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)](https://www.abcam.com/ps/products/249/ab249334/Images/ab249334-410373-anti-dlc-a-antibody-epr11240-bsa-and-azide-free-western-blot.jpg "Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11240] to DLC-A - BSA and Azide free
- Suitable for: ICC, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-DLC-A antibody [EPR11240] - BSA and Azide free
See all DLC-A primary antibodies -
Description
Rabbit monoclonal [EPR11240] to DLC-A - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human DLC-A.
Database link: Q9Y6G9 -
General notes
Ab249334 is the carrier-free version of ab157468. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249334 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11240 -
Isotype
IgG
Images
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Western blot - Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)All lanes : Anti-DLC-A antibody [EPR11240] (ab157468) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Fetal brain tissue lysate
Lane 4 : Fetal heart tissue lysate
Lane 5 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDaThis data was developed using ab157468, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)](https://www.abcam.com/ps/products/249/ab249334/Images/ab249334-4-anti-dlc-a-antibody-epr11240-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)This data was developed using ab157468, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling DLC-A with ab157468 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)](https://www.abcam.com/ps/products/249/ab249334/Images/ab249334-3-anti-dlc-a-antibody-epr11240-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)This data was developed using ab157468, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DLC-A with ab157468 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)](https://www.abcam.com/ps/products/249/ab249334/Images/ab249334-2-anti-dlc-a-antibody-epr11240-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334) -
![Western blot - Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)](https://www.abcam.com/ps/products/249/ab249334/Images/ab249334-398095-anti-dlc-a-antibody-epr11240-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)Anti-DLC-A antibody [EPR11240] (ab157468) at 1/1000 dilution + immunoprecipitation pellet from fetal brain lysate
Secondary
HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.
Predicted band size: 57 kDaThis data was developed using ab157468, the same antibody clone in a different buffer formulation.
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![Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)](https://www.abcam.com/ps/products/249/ab249334/Images/ab249334-6-benefits-of-recombinant-antibodies.png)
Anti-DLC-A antibody [EPR11240] - BSA and Azide free (ab249334)