Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Diphtheria Toxin antibody [IGX3492] (ab209329)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Human monoclonal [IGX3492] to Diphtheria Toxin
  • Suitable for: WB

Overview

  • Product name

    Anti-Diphtheria Toxin antibody [IGX3492]
    See all Diphtheria Toxin primary antibodies

  • Description

    Human monoclonal [IGX3492] to Diphtheria Toxin

  • Host species

    Human

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Diphtheria Toxin (mutated G52 E) full length protein

  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant antibody. 
    Human monoclonal antibody.

    For recommended secondary antibodies -

    Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 488) - ab200622
    Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 647) - ab200623
    Rabbit monoclonal Anti-Human IgG1 H&L (Biotin) - ab201485
    Rabbit Anti-Human IgG H&L (HRP)  - ab6759

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    IGX3492

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-Diphtheria Toxin antibody [IGX3492] (ab209329)
    Western blot - Anti-Diphtheria Toxin antibody [IGX3492] (ab209329)
    Anti-Diphtheria Toxin antibody [IGX3492] (ab209329) at 1 µg/ml + Native Diphtheria Toxin (mutated G52E) protein (ab188505) at 0.1 µg

    Secondary
    HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa


    Exposure time: 5 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209329 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
  • Anti-Diphtheria Toxin antibody [IGX3492] (ab209329)
    Anti-Diphtheria Toxin antibody [IGX3492] (ab209329)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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