Anti-DIO2 antibody (ab77779)
Key features and details
- Rabbit polyclonal to DIO2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DIO2 antibody
See all DIO2 primary antibodies -
Description
Rabbit polyclonal to DIO2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Goat, Cow, Pig, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to DIO2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab87369) -
Positive control
- This antibody gave a positive signal in the following human tissue lysates: Human Heart and Placenta, Mouse retina, P0 Whole Brain and Rat Whole Brain. This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab77779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Function
Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. -
Tissue specificity
Heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain. -
Sequence similarities
Belongs to the iodothyronine deiodinase family. -
Post-translational
modificationsUbiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 494548 Cow
- Entrez Gene: 1734 Human
- Entrez Gene: 13371 Mouse
- Entrez Gene: 414379 Pig
- Entrez Gene: 65162 Rat
- Omim: 601413 Human
- SwissProt: Q5I3B2 Cow
- SwissProt: Q92813 Human
see all -
Alternative names
- 5DII antibody
- D2 antibody
- Deiodinase, iodothyronine, type II antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DIO2 antibody (ab77779)
ICC/IF image of ab77779 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77779 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-DIO2 antibody (ab77779)All lanes : Anti-DIO2 antibody (ab77779) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Additional bands at: 105 kDa, 55 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-DIO2 antibody (ab77779)All lanes : Anti-DIO2 antibody (ab77779) at 1 µg/ml
Lane 1 : Mouse Retina Tissue Lysate
Lane 2 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Protocols
Datasheets and documents
References (2)
ab77779 has been referenced in 2 publications.
- Chen J et al. Chronic exposure to microcystin-LR reduces thyroid hormone levels by activating p38/MAPK and MEK/ERK signal pathway. Ecotoxicol Environ Saf 173:142-148 (2019). PubMed: 30771657
- Li M et al. Loss of selenocysteine insertion sequence binding protein 2 suppresses the proliferation, migration/invasion and hormone secretion of human trophoblast cells via the PI3K/Akt and ERK signaling pathway. Placenta 55:81-89 (2017). PubMed: 28623977
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DIO2 antibody (ab77779)
ICC/IF image of ab77779 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77779 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-DIO2 antibody (ab77779)All lanes : Anti-DIO2 antibody (ab77779) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Additional bands at: 105 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
-
Western blot - Anti-DIO2 antibody (ab77779)All lanes : Anti-DIO2 antibody (ab77779) at 1 µg/ml
Lane 1 : Mouse Retina Tissue Lysate
Lane 2 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
