Anti-DHX33 antibody (ab72451)
Key features and details
- Rabbit polyclonal to DHX33
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DHX33 antibody
See all DHX33 primary antibodies -
Description
Rabbit polyclonal to DHX33 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
A synthetic peptide corresponding to a region between residues 650 and the C terminus (residue 707) of human DHX33.
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Positive control
- Hela whole cell lysate; 293T cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified using an epitope specific to DHX33 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DHX33 antibody (ab72451)All lanes : Anti-DHX33 antibody (ab72451) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Observed band size: 79 kDa
Additional bands at: 200 kDa, 31 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Immunoprecipitation - Anti-DHX33 antibody (ab72451)Immunoprecipitation of DHX33 from 1mg whole HeLa cells lysate by 3 ug ab72451 followed by WB (20% of IP loaded):
Lane 1: ab72451 precipitate
Lane 2: Control IgG
For blotting immunoprecipitated DHX33, ab72451 was used at 1 ug/ml.
Detection: Chemiluminescence with an exposure time of 3 minutes.
