Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dhh antibody [EPR23386-212] (ab270453)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23386-212] to Dhh
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Dhh antibody [EPR23386-212]
    See all Dhh primary antibodies

  • Description

    Rabbit monoclonal [EPR23386-212] to Dhh

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human testis and Human colon tissue lysates; Recombinant Human Desert Hedgehog/Dhh protein. IHC-P: Human testis tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23386-212

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)
    Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)
    Anti-Dhh antibody [EPR23386-212] (ab270453) at 1/1000 dilution + Recombinant Human Desert Hedgehog/Dhh protein (aa 23-198) 20ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 44 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST   

    Exposure time: 10 seconds

  • Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)
    Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)
    All lanes : Anti-Dhh antibody [EPR23386-212] (ab270453) at 1/1000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Human colon tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 19 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature.  Band at 19kDa could be DHH N-terminal fragment (PMID: 31018998).  

    The expression profile observed is consistent with what has been described in the literature(PMID:22132805).  

    Exposure time: 3 minutes

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dhh antibody [EPR23386-212] (ab270453)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dhh antibody [EPR23386-212] (ab270453)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Dhh with ab270453 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human testis (PMID: 22132805). The section was incubated with ab270453 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Anti-Dhh antibody [EPR23386-212] (ab270453)
    Anti-Dhh antibody [EPR23386-212] (ab270453)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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