Anti-Desmin antibody [SP138] - C-terminal (ab227651)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP138] to Desmin - C-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Desmin antibody [SP138] - C-terminal
See all Desmin primary antibodies -
Description
Rabbit monoclonal [SP138] to Desmin - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseIHC-P MouseRatHumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human skeletal muscle, colon, esophagus, uterus endometrium, Mouse stomach, and Rat stomach tissue; WB: Human skeletal muscle tissue lysate; ICC: C2C12 cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP138 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling Desmin with ab227651 at 1/100 dilution (1.32 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling Desmin with ab227651 at 1/100 dilution (1.32 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Desmin with ab227651 at 1/100 dilution (1.32 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling Desmin with ab227651 at 1/100 dilution (1.32 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of C2C12 (mouse myoblasts myoblast) cells labeling Desmin with purified ab227651 at 1/100 (1.3µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-Desmin antibody [SP138] - C-terminal (ab227651) at 1/100 dilution + Human skeletal muscle tissue lysate
Predicted band size: 54 kDa
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Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Desmin using ab227651 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for Desmin using ab227651 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human esophagus tissue stained for Desmin using ab227651 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human uterus endometrium tissue stained for Desmin using ab227651 at 1/100 dilution in immunohistochemical analysis.
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