Anti-Desmin antibody (ab227224)
Key features and details
- Rabbit polyclonal to Desmin
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Desmin antibody
See all Desmin primary antibodies -
Description
Rabbit polyclonal to Desmin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Dog, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human Desmin (internal sequence). The exact sequence is proprietary.
Database link: P17661 -
Positive control
- WB: Rat2, RD, A549 and HCT116 whole cell lysate; mouse muscle tissue lysate. IHC-P: Mouse smooth muscle tissue and skeletal muscle tissue; H1299 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (ab227224)
Paraffin-embedded mouse smooth muscle tissue stained for Desmin with ab227224 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Desmin antibody (ab227224)Anti-Desmin antibody (ab227224) at 1/1000 dilution + Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 54 kDa10% SDS-PAGE
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Western blot - Anti-Desmin antibody (ab227224)Anti-Desmin antibody (ab227224) at 1/500 dilution + RD (human rhabdomyosarcoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 54 kDa10% SDS-PAGE
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Western blot - Anti-Desmin antibody (ab227224)All lanes : Anti-Desmin antibody (ab227224) at 1/3000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 54 kDa10% SDS-PAGE
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Western blot - Anti-Desmin antibody (ab227224)Anti-Desmin antibody (ab227224) at 1/1000 dilution + Mouse muscle tissue lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 54 kDa10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (ab227224)Paraffin-embedded mouse skeletal muscle tissue stained for Desmin with ab227224 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (ab227224)Paraffin-embedded NCI-H1299 (human lung carcinoma cell line) xenograft tissue stained for Desmin with ab227224 at 1/500 dilution in immunohistochemical analysis.
