Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5029(2)] to Delta Opioid Receptor
Suitable for: ICC, WB, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Delta Opioid Receptor antibody [EPR5029(2)]
See all Delta Opioid Receptor primary antibodies
Description

Rabbit monoclonal [EPR5029(2)] to Delta Opioid Receptor
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human Delta Opioid Receptor aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P41143
Positive control
- WB: Human brain, Mouse spleen, Rat spleen, and Mouse brain lysates\t\t\tICC/IF: SH-SY5Y cells. Flow Cyt: U-87 MG cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5029(2)
Isotype

IgG
Research areas

Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

All lanes : Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/2000 dilution (Purified)

Lane 1 : Human brain lysates
Lane 2 : Mouse spleen lysates
Lane 3 : Rat spleen lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 40 kDa
Observed band size: 40 kDa
Immunocytochemistry - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Delta Opioid Receptor with purified ab176324 at 1/100 dilution (10 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/10000 dilution (Purified) + Mouse brain lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 40 kDa
Observed band size: 40 kDa
Flow Cytometry - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

Flow Cytometry analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling Delta Opioid Receptor with purified ab176324 at 1/150 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

All lanes : Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/1000 dilution

Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal brain lysate
Lane 3 : U87-MG lysate
Lane 4 : HUVEC lysate
Lane 5 : SH-SY5Y lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 40 kDa
Immunocytochemistry - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling Delta Opioid Receptor at 1/500. Cells were fixed with 100% Methanol. An ab150077 AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

Control: primary antibody (1/500) and secondary antibody, ab150077 AlexaFluor®488 Goat anti-Rabbit secondary IgG (1/1000).
Flow Cytometry - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

Flow cytometric analysis of U87-MG cells labeling Delta Opioid Receptor using ab176324 at a 1/10 dilution (red) or a rabbit IgG control (green).
Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

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