Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5029(2)] to Delta Opioid Receptor
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Delta Opioid Receptor antibody [EPR5029(2)]
    See all Delta Opioid Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR5029(2)] to Delta Opioid Receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Delta Opioid Receptor aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P41143

  • Positive control

    • WB: Human brain, Mouse spleen, Rat spleen, and Mouse brain lysates\t\t\tICC/IF: SH-SY5Y cells. Flow Cyt: U-87 MG cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5029(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    All lanes : Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/2000 dilution (Purified)

    Lane 1 : Human brain lysates
    Lane 2 : Mouse spleen lysates
    Lane 3 : Rat spleen lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Immunocytochemistry - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Immunocytochemistry - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Delta Opioid Receptor with purified ab176324 at 1/100 dilution (10 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/10000 dilution (Purified) + Mouse brain lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Flow Cytometry - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Flow Cytometry - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Flow Cytometry analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling Delta Opioid Receptor with purified ab176324 at 1/150 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Western blot - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    All lanes : Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324) at 1/1000 dilution

    Lane 1 : Human cerebellum lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : U87-MG lysate
    Lane 4 : HUVEC lysate
    Lane 5 : SH-SY5Y lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa

  • Immunocytochemistry - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Immunocytochemistry - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

    Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling Delta Opioid Receptor at 1/500. Cells were fixed with 100% Methanol. An ab150077 AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

     

    Control: primary antibody (1/500) and secondary antibody, ab150077 AlexaFluor®488 Goat anti-Rabbit secondary IgG (1/1000).

  • Flow Cytometry - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Flow Cytometry - Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

    Flow cytometric analysis of U87-MG cells labeling Delta Opioid Receptor using ab176324 at a 1/10 dilution (red) or a rabbit IgG control (green).

  • Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)
    Anti-Delta Opioid Receptor antibody [EPR5029(2)] (ab176324)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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