Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24148-58] to DDX4 / MVH - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free
    See all DDX4 / MVH primary antibodies

  • Description

    Rabbit monoclonal [EPR24148-58] to DDX4 / MVH - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human testis tissue lysate; Mouse testis tissue lysate; rat testis tissue lysate. IHC-P: Human testis and overy tissue; Mouse testis and ovary tissue; Rat testis and ovary tissue. IHC-Fr: Mouse testis tissue; Rat testis tissue.

  • General notes

    ab277638 is the carrier-free version of ab270534. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277638 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)

    This data was developed using ab270534, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in spermatogonia and spermatocytes of human testis (PMID:26444630). The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)

    This data was developed using ab270534, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse testis tissue labeling DDX4 / MVH with ab270534 at 1/250 (1.824 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse testis is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    All lanes : Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534) at 1/1000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Human spleen tissue lysate
    Lane 3 : Human colon tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 79 kDa
    Observed band size: 79 kDa



    This data was developed using ab270534, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Human spleen, Human colon (PMID: 10920202).

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)

    This data was developed using ab270534, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in spermatogonia and spermatocytes of mouse testis. The section was incubated with ab270534 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    All lanes : Anti-DDX4 / MVH antibody [EPR24148-58] (ab270534) at 1/1000 dilution

    Lane 1 : Rat testis tissue lysate
    Lane 2 : Mouse testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 79 kDa
    Observed band size: 79 kDa



    This data was developed using ab270534, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: lane 1: 10 seconds lane 2: 7 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)

    This data was developed using ab270534, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human ovary tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in oocytes of human ovary (PMID:26444630).The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    Immunohistochemistry (Frozen sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)

    This data was developed using ab270534, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat testis tissue labeling DDX4 / MVH with ab270534 at 1/250 (1.824 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat testis is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)

    This data was developed using ab270534, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in oocytes of mouse ovary. The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)

    This data was developed using ab270534, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in spermatogonia and spermatocytes of rat testis.The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)

    This data was developed using ab270534, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat ovary tissue labeling DDX4 / MVH with ab270534 at 1/2000 (0.228 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in oocytes of rat ovary. The section was incubated with ab270534 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)
    Anti-DDX4 / MVH antibody [EPR24148-58] - BSA and Azide free (ab277638)

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