Anti-DDX28 antibody (ab70821)
Key features and details
- Rabbit polyclonal to DDX28
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDX28 antibody
See all DDX28 primary antibodies -
Description
Rabbit polyclonal to DDX28 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 500 and the C-terminus (residue 540) of human DDX28 (NP_060850.1).
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Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DDX28 antibody (ab70821)All lanes : Anti-DDX28 antibody (ab70821) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - Anti-DDX28 antibody (ab70821)Detection of Human DDX28 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70821 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of DDX28 was performed using ab70821 at 1 µg/ml.
