Anti-DDIT3 (phospho S30) antibody (ab63392)
Key features and details
- Rabbit polyclonal to DDIT3 (phospho S30)
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDIT3 (phospho S30) antibody
See all DDIT3 primary antibodies -
Description
Rabbit polyclonal to DDIT3 (phospho S30) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of GADD153 only when phosphorylated at serine 30. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human -
Immunogen
A synthesized phosphopeptide derived from human GADD153 around the phosphorylation site of serine 30 (VLSPSD).
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Positive control
- A549 cells and human breast carcinoma tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunofluorescence analysis of A549 cells, using ab63392 at 1:500 dilution.
Left image untreated.Right image treated with phospho peptide. -
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab63392 at 1:50 dilution.
Left image untreated.Right image treated with phospho peptide.