Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9811] to DDB2
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-DDB2 antibody [EPR9811]
See all DDB2 primary antibodies
Description

Rabbit monoclonal [EPR9811] to DDB2
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, SH-SY5Y and Raji cell lysates; IHC-P: Human stomach carcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9811
Isotype

IgG
Research areas

Western blot - Anti-DDB2 antibody [EPR9811] (ab181136)

Lane 1 : Anti-DDB2 antibody [EPR9811] (ab181136) at 1/2000 dilution
Lane 2 : Anti-DDB2 antibody [EPR9811] (ab181136) at 1/2000 dilution (Purified)

Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 48 kDa

40kDa band might be an isomer of DNA damage-binding protein 2 (Isomer D3).
Western blot - Anti-DDB2 antibody [EPR9811] (ab181136)

Anti-DDB2 antibody [EPR9811] (ab181136) at 1/1000 dilution + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 48 kDa

40kDa band might be an isomer of DNA damage-binding protein 2 (Isomer D3).
Western blot - Anti-DDB2 antibody [EPR9811] (ab181136)

All lanes : Anti-DDB2 antibody [EPR9811] (ab181136) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : DDB2 knockout HeLa cell lysate
Lane 3 : Raji cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 48 kDa
Observed band size: 48 kDa

Lanes 1-3: Merged signal (red and green). Green - ab181136 observed at 48 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab181136 Anti-DDB2 antibody [EPR9811] was shown to specifically react with DDB2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265115 (knockout cell lysate ab257177) was used. Wild-type and DDB2 knockout samples were subjected to SDS-PAGE. ab181136 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDB2 antibody [EPR9811] (ab181136)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue sections labeling DDB2 with Purified ab181136 at 1/2500 dilution (0.25 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Anti-DDB2 antibody [EPR9811] (ab181136)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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