Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1599Y] to Cytokeratin 4 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IP, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free
    See all Cytokeratin 4 primary antibodies

  • Description

    Rabbit monoclonal [EP1599Y] to Cytokeratin 4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IP, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: HeLa cells IP: HaCaT whole cell lysate.

  • General notes

    Ab247316 is the carrier-free version of ab51599. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247316 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
    This data was developed using ab51599, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human cervical carcinoma tissue using ab51599 (1/100). Goat anti-rabbit HRP (1/2000) used for secondary staining. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
    Immunocytochemistry - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
    This data was developed using ab51599, the same antibody clone in a different buffer formulation.ICC/IF image of ab51599 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab51599 at 1/100 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
  • Western blot - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
    Western blot - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
    Anti-Cytokeratin 4 antibody [EP1599Y] (ab51599) at 1/1000 dilution + A431 cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 57 kDa
    Observed band size: 59 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab51599, the same antibody clone in a different buffer formulation.

     

  • Flow Cytometry - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
    Flow Cytometry - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
    This data was developed using ab51599, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 4 with purified ab51599 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
  • Immunoprecipitation - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
    Immunoprecipitation - Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)

    This data was developed using ab51599, the same antibody clone in a different buffer formulation.
    Purified ab51599 at 1/50 dilution (2µg) immunoprecipitating Cytokeratin 4 in HaCaT whole cell lysate.
    Lane 1 (input): HaCaT (Human skin keratinocyte) whole cell lysate 10µg
    Lane 2 (+): ab51599 + HaCaT whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51599 in HaCaT whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 59 kDa

  • Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)
    Anti-Cytokeratin 4 antibody [EP1599Y] - BSA and Azide free (ab247316)

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