Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸ 10 µl 150 768 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cystatin C antibody [EPR4413] (ab109508)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4413] to Cystatin C
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-Cystatin C antibody [EPR4413]
See all Cystatin C primary antibodies
Description

Rabbit monoclonal [EPR4413] to Cystatin C
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant protein fragment corresponding to residues in Human Cystatin C.
Positive control
- WB: Human testis, Caco2, U20S, HeLa, HepG2, and U87-MG lysates. IHC-P: Human brain tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4413
Isotype

IgG
Research areas

Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: CST3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Caco2 whole cell lysate (20 µg)
Lane 4: U20S whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab109508 observed at 15 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab109508 was shown to specifically react with CST3 when CST3 knockout samples were used. Wild-type and CST3 knockout samples were subjected to SDS-PAGE. Ab109508 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Cystatin C antibody [EPR4413] (ab109508)

All lanes : Anti-Cystatin C antibody [EPR4413] (ab109508) at 1/10000 dilution

Lane 1 : Human testis lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : U87-MG cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 15 kDa
Observed band size: 13 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin C antibody [EPR4413] (ab109508)

Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109508 at adilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Cystatin C antibody [EPR4413] (ab109508)

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