Anti-Cystathionase/CTH antibody [EPR15468] - N-terminal (ab189916)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15468] to Cystathionase/CTH - N-terminal
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Cystathionase/CTH antibody [EPR15468] - N-terminal
See all Cystathionase/CTH primary antibodies -
Description
Rabbit monoclonal [EPR15468] to Cystathionase/CTH - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa and K562 cell lysates.
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General notes
This product was previously labelled as Cystathionase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15468 -
Isotype
IgG -
Research areas
Images
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Cystathionase/CTH knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: K562 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab189916 observed at 45 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab189916 was shown to recognize Cystathionase/CTH in wild type cells as signal was lost at the expected MW in Cystathionase/CTH knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Cystathionase/CTH knockout samples were subjected to SDS-PAGE. Ab189916 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Anti-Cystathionase/CTH antibody [EPR15468] - N-terminal (ab189916) at 0.01 µg/ml + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 45 kDa
Exposure time: 1 minuteBlocking and diluting buffer: 5% NFDM/TBST.
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Immunoprecipitation of Cystathionase/CTH from HeLa cell lysate using ab189916 at 1/50 or 1X PBS (negative control) (lane 1). Immunostained with ab189916 at 1/1500 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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