Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CYP46A1 antibody [1A7] - BSA and Azide free (ab255757)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1A7] to CYP46A1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CYP46A1 antibody [1A7] - BSA and Azide free
    See all CYP46A1 primary antibodies

  • Description

    Mouse monoclonal [1A7] to CYP46A1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Human CYP46A1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9Y6A2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Mouse and rat brain lysate. IHC-P: Mouse cerebrum tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255757 is the carrier-free version of ab244241. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Images

  • Western blot - Anti-CYP46A1 antibody [1A7] - BSA and Azide free (ab255757)
    Western blot - Anti-CYP46A1 antibody [1A7] - BSA and Azide free (ab255757)
    All lanes : Anti-CYP46A1 antibody [1A7] (ab244241) at 0.43 µg/ml

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 56 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244241).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP46A1 antibody [1A7] - BSA and Azide free (ab255757)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP46A1 antibody [1A7] - BSA and Azide free (ab255757)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling CYP46 with ab244241 at 8.6µg/ml followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse cerebrum. The section was incubated with ab244241 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244241).

  • Anti-CYP46A1 antibody [1A7] - BSA and Azide free (ab255757)
    Anti-CYP46A1 antibody [1A7] - BSA and Azide free (ab255757)

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