Anti-Cyclophilin B antibody [M019] (ab253289)
![Anti-Cyclophilin B antibody [M019] (ab253289)](https://www.abcam.com/ps/products/253/ab253289/Images/ab253289-343898-AntiCyclophilin-B-antibody-M019-immunocytochemistry-a549-human.jpg "Anti-Cyclophilin B antibody [M019] (ab253289)")
Key features and details
- Mouse monoclonal [M019] to Cyclophilin B
- Suitable for: ICC/IF, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Cyclophilin B antibody [M019]
See all Cyclophilin B primary antibodies -
Description
Mouse monoclonal [M019] to Cyclophilin B -
Host species
Mouse -
Specificity
Detects a 20 kDa band corresponding to the molecular mass of cyclophilin B on SDS-PAGE immunoblots of human LNCaP, MeWo, MCF7, and MDA-MB-231 cell lysates, as well as full length recombinant cyclophilin B, but does not detect recombinant cyclophilin A.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Other Immunogen Type within Human Cyclophilin B. The exact sequence is proprietary. Clone M019 was generated from a proprietary antigen related to cyclophilin B from the MCF7 breast cancer cell line.
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Positive control
- WB: LNCaP, MeWo, MCF7 and MDA-MB-231 cell lysates. Recombinant full length Cyclophilin B protein. ICC/IF: A549 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M019 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin B antibody [M019] (ab253289)
Methanol/Acetone (1:1) fixed, A549 (Human lung carcinoma cell line) cells labeling Cyclophilin B using ab253289 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
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![Western blot - Anti-Cyclophilin B antibody [M019] (ab253289)](https://www.abcam.com/ps/products/253/ab253289/Images/ab253289-343897-AntiCyclophilin-B-antibody-M019-western-blot-LNCaP-MeWo-MCF7-MDAMB231--human.jpg)
Western blot - Anti-Cyclophilin B antibody [M019] (ab253289)All lanes : Anti-Cyclophilin B antibody [M019] (ab253289) at 1/1000 dilution
Lane 1 : LNCaP (Human prostate cancer cell line) cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Lane 3 : MDA-MB-231 (Human cell line) cell lysate
Lane 4 : MeWo (Human malignant melanoma cell line) cell lysate
Lane 5 : Recombinant full length Cyclophilin B protein
Lane 6 : Recombinant full length Cyclophilin A protein
Predicted band size: 24 kDaWestern blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hour at room temperature.