Anti-Cyclin D1 (phospho T286) + Cyclin D2 antibody [EPR22328-235] - BSA and Azide free (ab256538)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22328-235] to Cyclin D1 (phospho T286) + Cyclin D2 (phospho T286) - BSA and Azide free
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cyclin D1 (phospho T286) + Cyclin D2 antibody [EPR22328-235] - BSA and Azide free -
Description
Rabbit monoclonal [EPR22328-235] to Cyclin D1 (phospho T286) + Cyclin D2 (phospho T286) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blotmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab256538 is the carrier-free version of ab230818. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256538 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22328-235 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-Cyclin D1 (phospho T286) + Cyclin D2 antibody [EPR22328-235] - BSA and Azide free (ab256538)
Dot blot analysis using ab230818 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/100,000 dilution.
Lane 1: CCND1 non-phospho peptide (aa279-294)
Lane 2: CCND1 (phospho T286) peptide (aa284-294)
Lane 3: CCND2 (phospho T280) peptide (aa278-288
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230818).
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