Anti-Cyclin B1 (phospho S147) antibody (ab60986)
Key features and details
- Rabbit polyclonal to Cyclin B1 (phospho S147)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin B1 (phospho S147) antibody
See all Cyclin B1 primary antibodies -
Description
Rabbit polyclonal to Cyclin B1 (phospho S147) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Cyclin B1 (phospho S147). Immunogen range is aa 121-170.
Database link: P14635
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cyclin B1 (phospho S147) antibody (ab60986)All lanes : Anti-Cyclin B1 (phospho S147) antibody (ab60986) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells treated with UV light (15 mins).
Lane 2 : Extracts from Jurkat cells treated with UV light (15 mins) and the immunising phosphopeptide.
Predicted band size: 48 kDa
Observed band size: >48 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 (phospho S147) antibody (ab60986)Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab60986 at 1/50-1/100 dilution, with or without the immunising phosphopeptide.
