Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cyclin B1 antibody [V152] - BSA and Azide free (ab186926)

Key features and details

  • Mouse monoclonal [V152] to Cyclin B1 - BSA and Azide free
  • Suitable for: IHC-P, IHC (PFA fixed), ICC/IF, SDS-PAGE, IHC-FoFr, IHC-Fr, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Cyclin B1 antibody [V152] - BSA and Azide free
    See all Cyclin B1 primary antibodies

  • Description

    Mouse monoclonal [V152] to Cyclin B1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, IHC (PFA fixed), ICC/IF, SDS-PAGE, IHC-FoFr, IHC-Fr, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Fusion protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1 Wild type, HeLa and HEK-293T whole cell lysate, IHC-P: Human tonsil tissue.

  • General notes

    ab186926 is the carrier-free version of ab72. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab182842 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Cyclin B1 antibody [V152] - BSA and Azide free (ab186926)
    Western blot - Anti-Cyclin B1 antibody [V152] - BSA and Azide free (ab186926)

    Lanes:
    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Cyclin B1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HEK293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab72 observed at 55 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab72 detected the expected band for Cyclin B1 in wild type HAP1 cells and the band was not seen in Cyclin B1 knockout HAP1 cells. Wild-type and Cyclin B1 knockout samples were subjected to SDS-PAGE. Ab72 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab72).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [V152] - BSA and Azide free (ab186926)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 antibody [V152] - BSA and Azide free (ab186926)

    ab72 staining Cyclin B1 in human tonsil tissue - formalin-fixed paraffin-embedded section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab72, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab72).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Cyclin B1 antibody [V152] - BSA and Azide free (ab186926)

© 2021 Astana Biomed Group LLP. All rights reserved.