Anti-CYB561A3 antibody (ab185304)
Key features and details
- Rabbit polyclonal to CYB561A3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CYB561A3 antibody -
Description
Rabbit polyclonal to CYB561A3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human CYB561A3 aa 200 to the C-terminus.
Database link: Q8NBI2 -
Positive control
- IHC-P: Human stomach tissue; WB: Transfected HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CYB561A3 antibody (ab185304)All lanes : Anti-CYB561A3 antibody (ab185304) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : CYB561A3 over-expression - HEK-293T whole cell lysate, (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Predicted band size: 27 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYB561A3 antibody (ab185304)Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling CYB561A3 in the membrane and cytoplasm of glandular cells with ab185304 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.