Anti-CXCL2 antibody [EPR23331-159] - BSA and Azide free (ab275888)
![Anti-CXCL2 antibody [EPR23331-159] - BSA and Azide free (ab275888)](https://www.abcam.com/ps/products/275/ab275888/Images/ab275888-412173-anti-cxcl2-antibody-epr23331159-western-blot-organ.jpg "Anti-CXCL2 antibody [EPR23331-159] - BSA and Azide free (ab275888)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23331-159] to CXCL2 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Rat
Overview
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Product name
Anti-CXCL2 antibody [EPR23331-159] - BSA and Azide free
See all CXCL2 primary antibodies -
Description
Rabbit monoclonal [EPR23331-159] to CXCL2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP RatWB Rat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat lung organ (treated with 10 µg/ml Concanavalin A for 3 days) culture, supernatant ;Recombinant Rat CXCL2 protein. IP: Rat lung (organ treated with 10 µg/ml Concanavalin A for 3 days) culture, supernatant.
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General notes
ab275888 is the carrier-free version of ab275879. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275888 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23331-159 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CXCL2 antibody [EPR23331-159] - BSA and Azide free (ab275888)All lanes : Anti-CXCL2 antibody [EPR23331-159] (ab275879) at 1/1000 dilution
Lane 1 : Untreated rat lung organ culture, supernatant
Lane 2 : Rat lung organ (treated with 10/ml Concanavalin A for 3 days) culture, supernatant
Lysates/proteins at 10 µl per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?This data was developed using ab275879, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
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![Immunoprecipitation - Anti-CXCL2 antibody [EPR23331-159] - BSA and Azide free (ab275888)](https://www.abcam.com/ps/products/275/ab275888/Images/ab275888-412227-anticxcl2antibodyepr23331159immunoprecipitationratlungorgantreatedwith10ugmlconcanavalinafor3daysculturesupernatant10ul.jpg)
Immunoprecipitation - Anti-CXCL2 antibody [EPR23331-159] - BSA and Azide free (ab275888)This data was developed using ab275879, the same antibody clone in a different buffer formulation.
CXCL2 was immunoprecipitated from 0.35 mg Rat lung (organ treated with 10 ug/ml Concanavalin A for 3 days) culture supernatant with ab275879 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275879 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat lung organ treated with 10 ug/ml Concanavalin A for 3 days culture supernatant 10 μl
Lane 2: ab275879 IP in Rat lung (organ treated with 10 ug/ml Concanavalin A for 3 days) culture supernatant
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275879 in rat lung organ treated with 10 ug/ml Concanavalin A for 3 days culture supernatant
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 seconds.
This blot was developed using a higher sensitivity ECL substrate.
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![Western blot - Anti-CXCL2 antibody [EPR23331-159] - BSA and Azide free (ab275888)](https://www.abcam.com/ps/products/275/ab275888/Images/ab275888-412172-anti-cxcl2-antibody-epr23331159-western-blot-protein.jpg)
Western blot - Anti-CXCL2 antibody [EPR23331-159] - BSA and Azide free (ab275888)Anti-CXCL2 antibody [EPR23331-159] (ab275879) at 1/1000 dilution + Recombinant Rat CXCL2 protein, 10 ng
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?This data was developed using ab275879, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds.
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![Anti-CXCL2 antibody [EPR23331-159] - BSA and Azide free (ab275888)](https://www.abcam.com/ps/products/275/ab275888/Images/ab275888-4-benefits-of-recombinant-antibodies.png)
Anti-CXCL2 antibody [EPR23331-159] - BSA and Azide free (ab275888)
