Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

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Anti-CXCL14 antibody [EPR22807-28] (ab264467)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22807-28] to CXCL14
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-CXCL14 antibody [EPR22807-28]
See all CXCL14 primary antibodies
Description

Rabbit monoclonal [EPR22807-28] to CXCL14
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293 transfected with CXCL14, Human skin, Rat skin, RAW 264.7, Mouse skin, Rat lung and Mouse lung lysates. ICC/IF: HEK-293T cells transfected with myc-tagged CXCL14 expression vector.Flow Cyt: HEK-293T cells transfected with myc-tagged CXCL14 expression vector.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22807-28
Isotype

IgG
Research areas

Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)

All lanes : Anti-CXCL14 antibody [EPR22807-28] (ab264467) at 1/1000 dilution

Lane 1 : Human skin lysate at 10 µg
Lane 2 : Rat skin lysate at 10 µg
Lane 3 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 4 : Mouse skin lysate at 20 µg
Lane 5 : Rat lung lysate at 20 µg
Lane 6 : Mouse lung lysate at 10 µg

Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 3-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 13 kDa
Observed band size: 14 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 15843547, 2602137, 28359053).

This blot was developed using a higher sensitivity ECL substrate.
Immunocytochemistry/ Immunofluorescence - Anti-CXCL14 antibody [EPR22807-28] (ab264467)

Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with myc-tagged CXCL14 expression vector) cells labeling CXCL14 with ab264467 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary secondary antibody at 1/1000 dilution (Green). The myc tag is also detected with a Myc-Tag (9B11) Mouse mAb (Alexa Fluor^® 647 Conjugate) (Red). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged CXCL14 expression vector. The nuclear counter stain is DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-CXCL14 antibody [EPR22807-28] (ab264467)

Flow cytometric analysis of [4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with myc-tagged CXCL14 expression vector) cells labeling CXCL14 with ab264467 at 1/500 dilution (Right) compared with a Isotype control details (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150079), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab264467 (Right). Then stained with anti-myc tag conjugated to Alexa Fluor^® 488.
Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)

All lanes : Anti-CXCL14 antibody [EPR22807-28] (ab264467) at 1/1000 dilution

Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293 transfected with CXCL14 (WT) expression vector containing a myc-His-tag®, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 13 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-CXCL14 antibody [EPR22807-28] (ab264467)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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