Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CXCL14 antibody [EPR22807-28] (ab264467)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22807-28] to CXCL14
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CXCL14 antibody [EPR22807-28]
    See all CXCL14 primary antibodies

  • Description

    Rabbit monoclonal [EPR22807-28] to CXCL14

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293 transfected with CXCL14, Human skin, Rat skin, RAW 264.7, Mouse skin, Rat lung and Mouse lung lysates. ICC/IF: HEK-293T cells transfected with myc-tagged CXCL14 expression vector.Flow Cyt: HEK-293T cells transfected with myc-tagged CXCL14 expression vector.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
    Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
    All lanes : Anti-CXCL14 antibody [EPR22807-28] (ab264467) at 1/1000 dilution

    Lane 1 : Human skin lysate at 10 µg
    Lane 2 : Rat skin lysate at 10 µg
    Lane 3 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
    Lane 4 : Mouse skin lysate at 20 µg
    Lane 5 : Rat lung lysate at 20 µg
    Lane 6 : Mouse lung lysate at 10 µg

    Secondary
    Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lanes 3-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 15843547, 2602137, 28359053).

    This blot was developed using a higher sensitivity ECL substrate.

  • Immunocytochemistry/ Immunofluorescence - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
    Immunocytochemistry/ Immunofluorescence - Anti-CXCL14 antibody [EPR22807-28] (ab264467)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with myc-tagged CXCL14 expression vector) cells labeling CXCL14 with ab264467 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary secondary antibody at 1/1000 dilution (Green). The myc tag is also detected with a Myc-Tag (9B11) Mouse mAb (Alexa Fluor® 647 Conjugate) (Red). Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged CXCL14 expression vector. The nuclear counter stain is DAPI (Blue).
    Secondary antibody only control: Used PBS instead of primary antibody, followed by  ab150077 AlexaFluor®488 Goat anti-Rabbit secondary secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
    Flow Cytometry - Anti-CXCL14 antibody [EPR22807-28] (ab264467)

    Flow cytometric analysis of [4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human embryonic kidney epithelial cell) (transfected with myc-tagged CXCL14 expression vector) cells labeling CXCL14 with ab264467 at 1/500 dilution (Right) compared with a Isotype control details (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150079), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab264467 (Right). Then stained with anti-myc tag conjugated to Alexa Fluor® 488.

  • Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
    Western blot - Anti-CXCL14 antibody [EPR22807-28] (ab264467)
    All lanes : Anti-CXCL14 antibody [EPR22807-28] (ab264467) at 1/1000 dilution

    Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 2 : HEK-293 transfected with CXCL14 (WT) expression vector containing a myc-His-tag®, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-CXCL14 antibody [EPR22807-28] (ab264467)
    Anti-CXCL14 antibody [EPR22807-28] (ab264467)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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