Anti-Cx36 antibody [2HCLC] (ab277766)
![Anti-Cx36 antibody [2HCLC] (ab277766)](https://www.abcam.com/ps/products/277/ab277766/Images/ab277766-1-antiConnexin36recombinantpolyclonalantibody2hclcICC.jpg "Anti-Cx36 antibody [2HCLC] (ab277766)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [2HCLC] to Cx36
- Suitable for: ICC, WB
- Reacts with: Human
Overview
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Product name
Anti-Cx36 antibody [2HCLC] -
Description
Rabbit recombinant multiclonal [2HCLC] to Cx36 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Cx36 aa 300-400.
Database link: Q9UKL4 -
Positive control
- ICC: MCF7 cells. WB: Jurkat whole cell lysate.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
2HCLC -
Isotype
IgG
Images
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Immunocytochemistry - Anti-Cx36 antibody (ab277766)
Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma cell line) cells stained with ab277766, using a: Alexa Fluor®488 Goat anti-Rabbit was used as secondary (green). b: DAPI was used to stain the nucleus (blue) and c: Alexa Fluor® 594 Phalloidin was used to stain actin (red). d: Composite image of cells showing cell membrane and junction localization of Connexin 36.
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Western blot - Anti-Cx36 antibody (ab277766)Anti-Cx36 antibody [2HCLC] (ab277766) at 2 µg/ml + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Predicted band size: 36 kDa
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![Anti-Cx36 antibody [2HCLC] (ab277766)](https://www.abcam.com/ps/products/277/ab277766/Images/ab277766-5-benefits-of-recombinant-antibodies.jpg)
Anti-Cx36 antibody [2HCLC] (ab277766)