Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR041F] to CTCF
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Mouse, Human

Product name

Anti-CTCF antibody [BLR041F]
See all CTCF primary antibodies
Description

Rabbit monoclonal [BLR041F] to CTCF
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Mouse Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human CTCF aa 650-700. The exact sequence is proprietary. NP_006556.1 and Gene ID 10664.
Database link: P49711
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR041F
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [BLR041F] (ab243897)

Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for CTCF using ab243897 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunocytochemistry - Anti-CTCF antibody [BLR041F] (ab243897)

Formalin-fixed, paraffin-embedded MCF7 (human breast adenocarcinoma cell line) cells labeling CTCF using ab243897 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody [BLR041F] (ab243897)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for CTCF using ab243897 at 1/100 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunoprecipitation - Anti-CTCF antibody [BLR041F] (ab243897)

CTCF was immunoprecipitated from 1.0 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab243897 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243897 at 1/1000 dilution.
Lane 1: ab243897 IP in HEK-293T whole cell lysate.

Lane 2: Control IgG in HEK-293T whole cell lysate.

Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot - Anti-CTCF antibody [BLR041F] (ab243897)

Western blot analysis using ab243897 at 1/1000 dilution.
Lane 1: HEK-293T whole cell lysate (50 µg).
Lane 2: MCF7 whole cell lysate (50 µg).
Lane 3: HepG2 whole cell lysate (50 µg).
Lane 4: A549 whole cell lysate (50 µg).
Lane 5: SW 620 whole cell lysate (50 µg).
Lane 6: SK whole cell lysate (50 µg).
Lane 7: Jurkat whole cell lysate (50 µg).
Lane 8: TCMK-1 whole cell lysate (50 µg).
Lane 9: NIH/3T3 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds.
Anti-CTCF antibody [BLR041F] (ab243897)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com