Anti-CTCF antibody (ab70303)
Key features and details
- Rabbit polyclonal to CTCF
- Suitable for: IHC-Fr, WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CTCF antibody
See all CTCF primary antibodies -
Description
Rabbit polyclonal to CTCF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseHumanIP HumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to a region between residues 650 and 700 of human CTCF (Peptide available as ab115042.)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab70303 was affinity purified using an epitope specific to CTCF immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CTCF antibody (ab70303)
Samples: Whole cell lysate (50 µg) from CT26.WT, 4T1, Renca, and TCMK-1 cells. ab70303 used at a dilution of 0.2 µg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTCF antibody (ab70303)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling CTCF with ab70303 at 1/100 (2µg/ml). Detection: DAB. -
Western blot - Anti-CTCF antibody (ab70303)All lanes : Anti-CTCF antibody (ab70303) at 0.03 µg/ml
Lane 1 : MCF7 whole cell lysate at 50 µg
Lane 2 : MCF7 whole cell lysate at 15 µg
Lane 3 : MCF7 whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Lane 6 : HeLa whole cell lysate at 50 µg
Exposure time: 30 seconds
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Immunoprecipitation - Anti-CTCF antibody (ab70303)Detection of Human CTCF by Western Blot of Immunprecipitate. Lane 1: MCF7 whole cell lysates immunoprecipitated using ab70302 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 2: MCF7 whole cell lysates immunoprecipitated using ab70303 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 3: MCF7 whole cell lysates immunoprecipitated using ab70304 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 4: control IgG was used for immunoprecipitation. All lanes: ab70303 at 0.03µg/ml. Detection: Chemiluminescence with exposure times of 10 seconds.
