Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRF antibody [EPR23255-162] - BSA and Azide free (ab272397)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23255-162] to CRF - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CRF antibody [EPR23255-162] - BSA and Azide free
    See all CRF primary antibodies

  • Description

    Rabbit monoclonal [EPR23255-162] to CRF - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat and mouse hypothalamus tissue. IHC-Fr: Rat and mouse hypothalamus tissue.

  • General notes

    ab272397 is the carrier-free version of ab272391. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] - BSA and Azide free (ab272397)
    Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] - BSA and Azide free (ab272397)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hypothalamus tissue labeling CRF with ab272391 at /100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 (2 µg/ml) dilution (Green). Positive staining on median eminence of rat hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondaryat 1000 (2 µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272391).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] - BSA and Azide free (ab272397)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] - BSA and Azide free (ab272397)

    Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling CRF with ab272391 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat hypothalamus is observed (PMID: 28883467). The section was incubated with ab272391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272391).

  • Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] - BSA and Azide free (ab272397)
    Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] - BSA and Azide free (ab272397)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hypothalamus tissue labeling CRF with ab272391 at /100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 (2 µg/ml) dilution (Green). Positive staining on median eminence of mouse hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondaryat 1000 (2 µg/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272391).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] - BSA and Azide free (ab272397)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] - BSA and Azide free (ab272397)

    Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling CRF with ab272391 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hypothalamus is observed (PMID: 23724107). The section was incubated with ab272391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272391).

  • Anti-CRF antibody [EPR23255-162] - BSA and Azide free (ab272397)
    Anti-CRF antibody [EPR23255-162] - BSA and Azide free (ab272397)

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