Anti-CRF antibody [EPR23255-162] (ab272391)
![Anti-CRF antibody [EPR23255-162] (ab272391)](https://www.abcam.com/ps/products/272/ab272391/Images/ab272391-4-anti-crf-antibody-epr23255162-immunohistochemistry-frozen-rat-hypothalamus.jpg "Anti-CRF antibody [EPR23255-162] (ab272391)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23255-162] to CRF
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-CRF antibody [EPR23255-162]
See all CRF primary antibodies -
Description
Rabbit monoclonal [EPR23255-162] to CRF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species 
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Rat and mouse hypothalamus tissue. IHC-Fr: Rat and mouse hypothalamus tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23255-162 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (ab272391)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hypothalamus tissue labeling CRF with ab272391 at /100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 (2 µg/ml) dilution (Green). Positive staining on median eminence of rat hypothalamus is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondaryat 1000 (2 µg/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (ab272391)](https://www.abcam.com/ps/products/272/ab272391/Images/ab272391-2-anti-crf-antibody-epr23255162-immunohistochemistry-rat-hypothalamus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (ab272391)Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling CRF with ab272391 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat hypothalamus is observed (PMID: 28883467). The section was incubated with ab272391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (ab272391)](https://www.abcam.com/ps/products/272/ab272391/Images/ab272391-3-anti-crf-antibody-epr23255162-immunohistochemistry-frozen-mouse-hypothalamus.jpg)
Immunohistochemistry (Frozen sections) - Anti-CRF antibody [EPR23255-162] (ab272391)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hypothalamus tissue labeling CRF with ab272391 at /100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1000 (2 µg/ml) dilution (Green). Positive staining on median eminence of mouse hypothalamus is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondaryat 1000 (2 µg/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (ab272391)](https://www.abcam.com/ps/products/272/ab272391/Images/ab272391-1-anti-crf-antibody-epr23255162-immunohistochemistry-mouse-hypothalamus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRF antibody [EPR23255-162] (ab272391)Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling CRF with ab272391 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hypothalamus is observed (PMID: 23724107). The section was incubated with ab272391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-CRF antibody [EPR23255-162] (ab272391)](https://www.abcam.com/ps/products/272/ab272391/Images/ab272391-5-benefits-of-recombinant-antibodies.png)
Anti-CRF antibody [EPR23255-162] (ab272391)
