Anti-CPEB1 antibody (ab73287)
Key features and details
- Rabbit polyclonal to CPEB1
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-CPEB1 antibody
See all CPEB1 primary antibodies -
Description
Rabbit polyclonal to CPEB1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatWB Rat
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Immunogen
A 14 amino acid peptide from near the amino terminus of human CPEB1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CPEB1 antibody (ab73287)Lane 1 : Anti-CPEB1 antibody (ab73287) at 1 µg/ml
Lane 2 : Anti-CPEB1 antibody (ab73287) at 2 µg/ml
All lanes : rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-CPEB1 antibody (ab73287)ICC/IF image of ab73287 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73287, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
