Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19467-36] to Coronin 1a/TACO - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free
    See all Coronin 1a/TACO primary antibodies

  • Description

    Rabbit monoclonal [EPR19467-36] to Coronin 1a/TACO - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon and spleen tissue; mouse spleen tissue; rat spleen tissue. ICC/IF: Jurkat cells. Flow cyt: Jurkat cells.

  • General notes

    ab243562 is the carrier-free version of ab203698 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab243562 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Coronin 1a

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Coronin 1a/TACO with ab203698 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on rat spleen (PMID: 17341475) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203698).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Coronin 1a/TACO with ab203698 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on mouse spleen (PMID: 17341475) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203698).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Coronin 1a/TACO with ab203698 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human spleen (PMID: 17341475) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203698).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Coronin 1a/TACO with ab203698 at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human colon (PMID: 17341475) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203698).

  • Flow Cytometry - Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)
    Flow Cytometry - Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293 (human epithelial cell line from embryonic kidney) (Left panel) and Jurkat (human T cell leukemia cell line from peripheral blood) (Right panel) cells labeling Coronin 1a/TACO with ab203698 at 1/500 dilution (red) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    Negative control: HEK-293 (PMID:17341475).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203698).

  • Immunocytochemistry/ Immunofluorescence - Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)
    Immunocytochemistry/ Immunofluorescence - Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Coronin 1a/TACO with ab203698 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Jurkat cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.

    Control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: HEK-293 (PMID:17341475).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203698).

  • Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)
    Anti-Coronin 1a/TACO antibody [EPR19467-36] - BSA and Azide free (ab243562)

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