Anti-Cordon bleu homolog antibody (ab254809)
Key features and details
- Rabbit polyclonal to Cordon bleu homolog
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cordon bleu homolog antibody -
Description
Rabbit polyclonal to Cordon bleu homolog -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human Cordon bleu homolog aa 150-300.
Database link: O75128 -
Positive control
- ICC/IF: CACO-2 cells. WB: siRNA control transfected HeLa cell lysate. IHC-P: Human parathyroid gland, colon, heart muscle and cerebral cortex tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Paraffin-embedded human heart muscle tissue stained for Cordon bleu homolog using ab254809 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon tissue stained for Cordon bleu homolog using ab254809 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human smooth muscle tissue stained for Cordon bleu homolog using ab254809 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human cerebral cortex tissue stained for Cordon bleu homolog using ab254809 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human parathyroid gland tissue stained for Cordon bleu homolog using ab254809 at 1/50 dilution in immunohistochemical analysis.
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Lanes 2-4 : Anti-Cordon bleu homolog antibody (ab254809) at 0.4 µg/ml
Lane 1 : DNA Ladder
Lane 2 : HeLa cells transfected with the control siRNA
Lane 3 : HeLa cells transfected with the siRNA probe 1
Lane 4 : HeLa cells transfected with the siRNA probe 2
Predicted band size: 76 kDa
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PFA fixed, Triton X-100 permeabilized CACO-2 (Human colorectal adenocarcinoma cell line) cells labeling Cordon bleu homolog using ab254809 at 4µg/ml (green) in ICC/IF.