Anti-COPE antibody [EPR12579] (ab180946)
![Anti-COPE antibody [EPR12579] (ab180946)](https://www.abcam.com/ps/products/180/ab180946/Images/ab180946-212868-ab1809461.jpg "Anti-COPE antibody [EPR12579] (ab180946)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12579] to COPE
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-COPE antibody [EPR12579]
See all COPE primary antibodies -
Description
Rabbit monoclonal [EPR12579] to COPE -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- MCF-7, PC-3, HeLa and A375 cell lysate. Human liver tissue. MCF-7 and A375 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12579 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-COPE antibody [EPR12579] (ab180946)All lanes : Anti-COPE antibody [EPR12579] (ab180946) at 1/5000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPE antibody [EPR12579] (ab180946)](https://www.abcam.com/ps/products/180/ab180946/Images/ab180946-212867-ab1809462.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPE antibody [EPR12579] (ab180946)Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling COPE using ab180946 at a 1/50 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-COPE antibody [EPR12579] (ab180946)](https://www.abcam.com/ps/products/180/ab180946/Images/ab180946-212866-ab1809463.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-COPE antibody [EPR12579] (ab180946)Immunofluorescent analysis of formaldehyde fixed MCF-7 cells labeling COPE using ab180946 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution. Counterstain DAPI.
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![Flow Cytometry - Anti-COPE antibody [EPR12579] (ab180946)](https://www.abcam.com/ps/products/180/ab180946/Images/ab180946-212865-ab1809464.jpg)
Flow Cytometry - Anti-COPE antibody [EPR12579] (ab180946)Flow Cytometry analysis of A375 cells labeling COPE using ab180946 at a 1/70 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
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![Anti-COPE antibody [EPR12579] (ab180946)](https://www.abcam.com/ps/products/180/ab180946/Images/ab180946-5-benefits-of-recombinant-antibodies.png)
Anti-COPE antibody [EPR12579] (ab180946)
